Journal: Oncology Letters

Article Title: Differential effect of psoralidin in enhancing apoptosis of colon cancer cells via nuclear factor-κB and B-cell lymphoma-2/B-cell lymphoma-2-associated X protein signaling pathways

doi: 10.3892/ol.2015.3861

Figure Lengend Snippet: Psoralidin treatment reduces the viability of SW480 cells. Values are expressed as the mean ± standard deviation. *P<0.05 and **P<0.01 compared with the control group.

Article Snippet: Cell line and culture The SW480 human colon cancer cell line was obtained from the Department of Oncology (Central Hospital of Jingzhou, Jingzhou, China).

Techniques: Standard Deviation, Control

Journal: Oncology Letters

Article Title: Differential effect of psoralidin in enhancing apoptosis of colon cancer cells via nuclear factor-κB and B-cell lymphoma-2/B-cell lymphoma-2-associated X protein signaling pathways

doi: 10.3892/ol.2015.3861

Figure Lengend Snippet: Psoralidin enhances cellular apoptosis of SW480 cells. Effect of psoralidin on the cellular apoptosis of SW480 cells examined by (A) flow cytometry and (B) DAPI staining assay (magnification, ×200). Values are expressed as the mean ± standard deviation. *P<0.05 and **P<0.01 compared with control group. FCM, flow cytometry.

Article Snippet: Cell line and culture The SW480 human colon cancer cell line was obtained from the Department of Oncology (Central Hospital of Jingzhou, Jingzhou, China).

Techniques: Flow Cytometry, Staining, Standard Deviation, Control

Journal: Oncology Letters

Article Title: Differential effect of psoralidin in enhancing apoptosis of colon cancer cells via nuclear factor-κB and B-cell lymphoma-2/B-cell lymphoma-2-associated X protein signaling pathways

doi: 10.3892/ol.2015.3861

Figure Lengend Snippet: Psoralidin treatment enhances caspase-3 activity of SW480 cells. Values are expressed as the mean ± standard deviation. *P<0.05 and **P<0.01 compared with control group.

Article Snippet: Cell line and culture The SW480 human colon cancer cell line was obtained from the Department of Oncology (Central Hospital of Jingzhou, Jingzhou, China).

Techniques: Activity Assay, Standard Deviation, Control

Journal: Oncology Letters

Article Title: Differential effect of psoralidin in enhancing apoptosis of colon cancer cells via nuclear factor-κB and B-cell lymphoma-2/B-cell lymphoma-2-associated X protein signaling pathways

doi: 10.3892/ol.2015.3861

Figure Lengend Snippet: Psoralidin reduces the NF-κB activity of SW480 cells. Values are expressed as the mean ± standard deviation. *P<0.05 and **P<0.01 compared with control group. NF-κB, nuclear factor-κB; OD, optical density.

Article Snippet: Cell line and culture The SW480 human colon cancer cell line was obtained from the Department of Oncology (Central Hospital of Jingzhou, Jingzhou, China).

Techniques: Activity Assay, Standard Deviation, Control

Journal: Oncology Letters

Article Title: Differential effect of psoralidin in enhancing apoptosis of colon cancer cells via nuclear factor-κB and B-cell lymphoma-2/B-cell lymphoma-2-associated X protein signaling pathways

doi: 10.3892/ol.2015.3861

Figure Lengend Snippet: Psoralidin inhibits Bcl-2 and enhances Bax protein expression in SW480 cells. (A and B) Effect of psoralidin on Bcl-2 protein expression of SW480 cells by western blotting assay and statistical analysis of Bcl-2 protein expression of SW480 cells. (C and D) Effects of psoralidin on Bax protein expression of SW480 cells by western blotting assay and statistical analysis of Bax proteins expression of SW480 cells. Values are expressed as the mean ± standard deviation. *P<0.05 and **P<0.01 compared with control group. Bcl-2, B-cell lymphoma-2; Bax, Bcl-2-associated X protein.

Article Snippet: Cell line and culture The SW480 human colon cancer cell line was obtained from the Department of Oncology (Central Hospital of Jingzhou, Jingzhou, China).

Techniques: Expressing, Western Blot, Standard Deviation, Control

Journal: Oncology Letters

Article Title: Differential effect of psoralidin in enhancing apoptosis of colon cancer cells via nuclear factor-κB and B-cell lymphoma-2/B-cell lymphoma-2-associated X protein signaling pathways

doi: 10.3892/ol.2015.3861

Figure Lengend Snippet: Bcl-2 inhibitor enhances the effect of psoralidin on SW480 cells. (A) Effect of Bcl-2 inhibitor on Bcl-2 protein expression of SW480 cells by western blotting assays; (B) statistical analysis of Bcl-2 protein expression of SW480 cells; and (C) Bcl-2 inhibitor enhances the effect of psoralidin on viability of SW480 cells. Values are expressed as the mean ± standard deviation. **P<0.01 compared with control group; ##P<0.01 compared with PSO (10) group. Bcl-2, B-cell lymphoma-2; PSO, psoralidin; ABT, ABT-737.

Article Snippet: Cell line and culture The SW480 human colon cancer cell line was obtained from the Department of Oncology (Central Hospital of Jingzhou, Jingzhou, China).

Techniques: Expressing, Western Blot, Standard Deviation, Control

Journal: Heliyon

Article Title: A novel lnc-LAMC2-1:1 SNP promotes colon adenocarcinoma progression by targeting miR-216a-3p/HMGB3

doi: 10.1016/j.heliyon.2022.e12342

Figure Lengend Snippet: The lnc-LAMC2-1:1 SNP is a sponge of miR-216a-3p . Bar graphs of (a) and (b) show the relative luciferase activity of vectors containing SW480. A dual-luciferase reporter assay was performed, and the co-transfection of lnc-LAMC2-1:1 SNP and miR-216a-3p reduced the luciferase activity.

Article Snippet: The SW480 cell line was obtained from COSMOBIO Company in China and was grown in DMEM supplemented with 10% FBS (GIBCO) and 1% antibiotics (50 U/mL penicillin and 50 μg/mL streptomycin) in a humidified atmosphere of 5% CO2 at 37 °C.

Techniques: Luciferase, Activity Assay, Reporter Assay, Cotransfection

Journal: Heliyon

Article Title: A novel lnc-LAMC2-1:1 SNP promotes colon adenocarcinoma progression by targeting miR-216a-3p/HMGB3

doi: 10.1016/j.heliyon.2022.e12342

Figure Lengend Snippet: The lnc-LAMC2-1:1 SNP positively regulates HMGB3 by sponging miR-126-3p (a) mRNA expression of HMGB3 in SW480 cells. Cells were transfected with mimics of NC, miR-126-3p mimics, ASO-NC, and ASO-miR-126-3p. (b) mRNA expression of HMGB3 in SW480 cells. Cells were transfected with pcDNA3.1, lnc-LAMC2-1:1 SNP , and lnc-LAMC2-1:1-wt. (c) Complementary sequences of HMGB3 and miR-126-3p in the StarBase database. (d) A dual-luciferase reporter assay was performed, and the co-transfection of miR-126-3p and HMGB3 reduced the luciferase activity.

Article Snippet: The SW480 cell line was obtained from COSMOBIO Company in China and was grown in DMEM supplemented with 10% FBS (GIBCO) and 1% antibiotics (50 U/mL penicillin and 50 μg/mL streptomycin) in a humidified atmosphere of 5% CO2 at 37 °C.

Techniques: Expressing, Transfection, Luciferase, Reporter Assay, Cotransfection, Activity Assay

Journal: Journal of Reports in Pharmaceutical Sciences

Article Title: Study of Oxidative Stress and Apoptotic Genes in SW480 Cell Lines Treated with Selenium Nanoparticles

doi: 10.5812/jrps-151064

Figure Lengend Snippet: Figure 2. Cell viability of selenium nanoparticles (SeNPs)-treated SW480 cell lines for 24h. Results are shown as ± SD. ns, not significant; ** P < 0.01; and *** P < 0.001 compared with untreated cells, (n = 3).

Article Snippet: The SW480 human colon cancer cell line was obtained from The Pasteur Institute (Tehran, Iran).

Techniques:

Journal: Journal of Reports in Pharmaceutical Sciences

Article Title: Study of Oxidative Stress and Apoptotic Genes in SW480 Cell Lines Treated with Selenium Nanoparticles

doi: 10.5812/jrps-151064

Figure Lengend Snippet: Figure 3. Reactive oxygen species (ROS) levels of selenium nanoparticles (SeNPs)-treated SW480 cell lines for 24h. Results are shown as mean ± SD. ** P < 0.01 and *** P < 0.001 compared with untreated cells, (n = 3).

Article Snippet: The SW480 human colon cancer cell line was obtained from The Pasteur Institute (Tehran, Iran).

Techniques:

Journal: Journal of Reports in Pharmaceutical Sciences

Article Title: Study of Oxidative Stress and Apoptotic Genes in SW480 Cell Lines Treated with Selenium Nanoparticles

doi: 10.5812/jrps-151064

Figure Lengend Snippet: Figure 4. Antioxidant enzymes activity of selenium nanoparticles (SeNPs)-treated SW480 cell lines for 24h, (n = 3). Results are shown as mean ± SD. * P < 0.05 ** P < 0.01, and *** P < 0.001.

Article Snippet: The SW480 human colon cancer cell line was obtained from The Pasteur Institute (Tehran, Iran).

Techniques: Activity Assay

Journal: Journal of Reports in Pharmaceutical Sciences

Article Title: Study of Oxidative Stress and Apoptotic Genes in SW480 Cell Lines Treated with Selenium Nanoparticles

doi: 10.5812/jrps-151064

Figure Lengend Snippet: Figure 5. Genes expression of Bax, Bcl-2, Bax/Bcl2 ratio, and P53 in selenium nanoparticles (SeNPs)-treated SW480 cell lines for 24h, (n = 3). Results are shown as mean ± SD. * P < 0.05 ** P < 0.01and *** P < 0.001.

Article Snippet: The SW480 human colon cancer cell line was obtained from The Pasteur Institute (Tehran, Iran).

Techniques: Expressing